商品编号


R0701S-250 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

250 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the cloned BfuAI gene from
Bacillus fusiformis
1083
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 3.1
-20
10X
Notes:
BfuAI requires two copies of its recognition sequence for cleavage to occur. Thus, the single BfuAI sites in pBR322 and pUC18 and 19 are resistant to cleavage. A 100-fold overdigestion cuts les than half of the DNA. Some other plasmid DNAs are also resistant to BfuAI cleavage.BfuAI cleaves plasmid DNAs more efficiently than BspMI.BfuAI is an isoschizomer of BspMI.Sites in some plasmid DNAs are cleaved at a slower rate than λ DNA.Star activity may result from a glycerol concentration of >5%