商品编号


R0109S-5,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

5,000 units

商品信息

Description:
FokI crystals (Ira Schildkraut and Lydia Dorner,
New England Biolabs
)
Product Source
An?
E. coli
strain that carries the FokI gene from
Flavobacterium okeanokoites
(IFO 12536).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
FokI can cleave between virtually any two nucleotides by constructing a complementary oligonucleotide to the sequence to be cleaved (Szybalski, W. (1985) Gene 40, 169-173, Podhajska, A. and Szybalski, W. (1985) Gene 40, 175-182).Overdigestions of >5 units of FokI per μg of DNA is not recommended.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.Star activity may result from a glycerol concentration of >5%