商品编号


R0513S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

500 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the AlwI gene from
Acinetobacter lwoffii
(R. Morgan).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
AlwI produces DNA fragments that have a single-base 5? extension which are more difficult to ligate than blunt-ended fragments.?This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%