商品编号


M0484S-100 reactions (50 μl vol)



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




100 reactions (50 μl vol)


Concentration

2X


商品信息

Description:
One
Taq
? Hot Start 2X Master Mix with Standard Buffer is an optimized blend of
Taq
and Deep Vent
R
? DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and
CDN
A products. The 3?→5? exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of
Taq
DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl
2
and other buffer components and st
ABI
lizers listed below, requiring only the addition of primers and DNA template for robust amplification
Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start 2X Master Mix with Standard Buffer. The presence of absence of an extended room temperature incubation does not affect performance. Amplicon sizes are indicated next to gel, and GC content is indicated below gel.
Marker
M is the 1 kb DNA Ladder (
NEB
#N3232 ).
??
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1 and an?
E. coli
strain that carries the Deep VentR DNA Polymerase gene from
Pyrococcus
species GB-D.
Notes:
Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with Standard Buffer are available separately.OneTaq Hot Start 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.OneTaq Hot Start 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.