NEB/BstZ17I-HF?/R3594S/1,000 units
克隆和表达
商品编号
R3594S-1,000 units
品牌
纽英伦(NEB)
公司
New England Biolabs
公司分类
Restriction Endonucleases
Concentration
20,000 units/ml
Size
1,000 units
商品信息
Description:
NEB
extensively performs quality controls on all standard and high-fidelity(HF?) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
Restriction Enzyme Competitor Study: Nuclease Contamination
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactionscontaining a fluorescent labeled single stranded, double stranded blunt,3’overhang or 5’ overhang containing oligonucleotides. The percent degradationis determined by capillary electrophoresis and peak analysis. The resolution isat the single nucleotide level.
Product Source
An
E. coli
strain that carries the cloned and modified BstZ17I gene from
Bacillus stearo
Thermo
philus
38M (Z. Chen).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Gel Lo
ADI
ng Dye, Purple (6X)
25
6X
Notes:
BstZ17I is an isoschizomer of BssNAI and Bst1107I.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
产品货号:639.2