商品编号


R0696S-1,250 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

1,250 units

商品信息

Description:
The intein encoding PI-SceI is present in the VMA ATPase gene
Saccharomyces cerevisiae
(1,5). The gene has been modified for independent expression in
E.?coli
using a T7 RNA polymerase expression system (2).
Product Source
An?
E. coli
strain that carries the VMA1 ATPase gene from
Saccharomyces cerevisiae
(J. Thorner).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
pBSvdeX XmnI-linearized Control Plasmid
500 μg/ml
Purified BSA
-20
10 mg/ml
NEB
uffer PI-SceI
-20
10X
Notes:
Supplied with plasmid DNA: XmnI-linearized pBSvdeX is supplied 0.5 mg/ml in 10 mM Tris-HCl (pH?8.0), 1 mM EDTA. Cleavage of this 3.7 kb plasmid with PI-SceI gives fragments of 2550 and 1150 base pairs Enzyme PropertiesHoming endonucleases do not have stringently-defined recognition sequences in the way that restriction enzymes do. That is, single base changes do not abolish cleavage but reduce its efficiency to variable extents. The precise boundary of required bases is generally not known. The recognition sequence listed is one site that is known to be recognized and cleaved.PI-SceI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis. To disrupt binding, add SDS to a final concentration of 0.5% or purify DNA before electrophoresis.