商品编号


M0485S-100 reactions (50 μl vol)



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




100 reactions (50 μl vol)


Concentration

2X


商品信息

Description:
One
Taq
? Hot Start 2X Master Mix with GC Buffer is an optimized blend of
Taq
and Deep Vent

? DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and
CDN
A products. The 3?→5? exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of
Taq
DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO
4,
?buffer components and st
ABI
lizers, requiring only the addition of primers and DNA template for robust amplification.
Amplification of a selection of sequences with varying GC content from human genomic DNA using OneTaq Hot Start 2X Master Mix with GC Buffer. The presence or absence of an extended room temperature incubation does not affect performance. Amplicon sizes are indicated next to gel, and GC content is indicated below gel.
Marker
M is the 1 kb DNA Ladder.
??
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1 and an?
E. coli
strain that carries the Deep VentR DNA Polymerase gene from
Pyrococcus
species GB-D.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
One
Taq
?High GC Enhancer
50%
Notes:
OneTaq Hot Start 2X Master Mix with GC Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.OneTaq Hot Start 2X Master Mix with GC Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with GC Buffer are available separately.