商品编号


R0707S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

1,000 units

商品信息

Description:
Nb.BtsI is a nicking endonuclease that cleaves only one strand of DNA on a double- stranded DNA substrate.
Product Source
An
?E. coli
?strain that expresses only the large subunit of the BtsI restriction gene from?
Bacillus
Thermo
glucosidasius
?(X.Pan).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
Nb.BtsI is very efficient. Under most conditions a one to two hour incubation with 1 ?l of enzyme and 1 ?g of DNA is recommended.?Nb.BtsI is 100% active at 55°C.?To run on an electrophoresis gel, add lo
ADI
ng dye containing a final concentration of 0.4% SDS.To see how this enzyme (along with other nicking enzymes) performs at different temperatures, please visit Effect of Various Temperatures of Different Nicking Endonucleases.