商品编号


R0157S-2,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

20,000 units/ml


Size

2,000 units

商品信息

Description:
Product Source
A
Streptomyces lividans
strain that carries the SacII gene from
Streptomyces achromogenes?
(
ATCC
12767).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
Certain SacII sites (e.g. one in the right arm of λ DNA) are resistant to cleavage. The reason particular SacII sites in λ DNA and ΦX174 DNA are cleaved at significantly lower rates than those found with other substrates is unclear at present.SacII needs to interact with two copies of its recognition sequence to cleave. As a result, substrates with single SacII sites are cleaved at a reduced rate.For more information and recommendations on working with enzymesrequiring multi-sites, please visit Restriction Enzyme Cleavage:‘single-site’ enzymes and ‘multi-site’ enzymes