商品编号


R0694S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

500 units

商品信息

Description:
I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI ispresent in the mitochondria of
Saccharomyces cerevisiae
.
Product Source
An
E. coli
?strain that carries the I-SceI mitochondrial gene from?
Saccharomyces cerevisiae
?(B. Dujon).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
pGPS2 NotI-linearized Control Plasmid
500 μg/ml
CutSmart? Buffer
-20
10X
Notes:
Homing endonucleases do not have stringently-defined recognition sequences in the way that restriction enzymes do. That is, single base changes do not abolish cleavage but reduce its efficiency to variable extents. The precise boundary of required bases is generally not known. The recognition sequence listed is one site that is known to be recognized and cleaved.Supplied with plasmid DNA. NotI-linearized pGPS2 is supplied at 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA. Cleavage of this 2499 bp plasmid with I-SceI gives fragments of 1518 and 981 base pairs.The enzyme retains full activity after at least 10 free-thaw cycles; the enzyme can be aliquoted and stored at –80C.