商品编号


R0158S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

1,000 units

商品信息

Description:
Product Source
An?
E. coli
strain that carries the HphI gene from
Haemophilus parahaemolyticus
(
ATCC
49700).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
It has been suggested that HphI may cleave at N9/N8 depending on the sequence between the recognition and cleavage sites (Kang, C. and Wu, C.-W. (1987) Nucl. Acids Res. 15, 2279-2294; Cho, S.-H. and Kang, C. (1990) Mol. Cells 1, 81?86.). Incubation of >?12 units for over 4 hours on ΦX174 DNA results in additional cleavage products. This has not yet been shown to occur on other DNAs. Low pH and high glycerol concentration enhance this activity.This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.This enzyme is blocked by dcm methylation. More information can be found at Dam-Dcm and CpG Methylation.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%