商品编号


R0637S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

2,000 units/ml


Size

500 units

商品信息

Description:
Product Source
An?
E. coli
?strain that carries the MmeI gene from
Methylophilus methylotrophus.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
S-adenosylmethionine (SAM)
-20
32 mM
CutSmart? Buffer
-20
10X
Notes:
Excess MmeI blocks cleavage.Reactions using MmeI should be done at or near stoichiometric concentrations.Complete cleavage occurs within 15 minutes at 37°C.Significant cleavage occurs on ice and at 50°C.MmeI activity is inhibited by high ionic strength (> 200 mM).SAM must be present in a reaction at a concentration of 50 ?M or higher for optimal cleavage.MmeI prefers two copies of its recognition sequence for cleavage to occur, with limited cleavage on plasmids with a single site.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.