商品编号


M0488S-100 reactions (50 μl vol)



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




100 reactions (50 μl vol)


Concentration

2X


商品信息

Description:
One
Taq
? Hot Start Quick-Load? 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of
Taq
and Deep Vent
R
? DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and
CDN
A products. The 3?→5? exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of
Taq
DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient Quick-Load master mix formulation contains dNTPs, MgCl
2
, buffer components and st
ABI
lizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. GC content is indicated below gel.
Marker
M is the 1 kb DNA Ladder (
NEB
#N3232 ).
??
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1 and an
E. coli
strain that carries the Deep VentR DNA Polymerase gene from
Pyrococcus
species GB-D.
Notes:
Product specifications for individual components in the OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer are available separately.OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°COneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.