商品编号


M0258S-200 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




200 units


Concentration

2,000 units/ml


商品信息

Description:
Deep Vent DNA Polymerase is the second high-fidelity
Thermo
philic DNA polymerase available from
New England Biolabs
. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3?→ 5? proofre
ADI
ng exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.
Deep Vent DNA Polymerase crystals (Ira Schildkraut and Rebecca Kucera,
New England Biolabs
, Inc.)
Amplification of Jurkat genomic DNA with Deep Vent DNA Polymerase.?Amplicon sizes are indicated below gel.
Marker
M is the 1 kb DNA Ladder (
NEB
#N3232 ).
Highlights
Isolated from a recombinant source
Supplied with 10X Reaction Buffer and 100 mMMgSO
4
High-Fidelity: 5X greater than
Taq
Extremely High
Thermo
st
ABI
lity: half-life of 23 hoursat 95°C
Difficult Templates: ideal for GC-rich or loopedsequences
Product Source
An?
E. coli?
strain that carries the Deep Vent DNA Polymerase gene from?
Pyrococcus?
species GB-D. The native organism was isolated from a su
BMA
rine thermal vent at 2,010 meters (1) and is able to grow at temperatures as high as 104°C.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Thermo
Pol
??
Reaction Buffer Pack
-20
10X
Notes:
BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as
NEB
#B9001. Acetylated BSA should not be used for primer extension reactions.The calculated half-life of Deep Vent DNA Polymerase at 95°C is 23 hours.Diluent D is also available (
NEB
# B8004). This diluent is recommended for making dilutions of Deep Vent Polymerase.Additional
Thermo
Pol Reaction Buffer packs for this product are also available (
NEB
# B9004). Each buffer pack contains 4 vials of 10X buffer (1.5 mls each) and 1 vial of 100 mM MgSO4.