商品编号


R0154S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

2,000 units/ml


Size

500 units

商品信息

Description:
Product Source
A
E. coli
strain that carries the HgaI gene from
Haemophilus gallinarum
(
ATCC
14385)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 1.1
-20
10X
Notes:
Of the over 3,000 known restriction endonucleases, HgaI is one of the few that produces extensions of more than 4?bases.Overdigestions with > 5 units of HgaI per ?g of DNA is not recommended.HgaI loses activity in the absence of substrate and is essentially inactive after one hour at 37°C.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%