商品编号


E0555S-50 reactions



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




50 reactions



商品信息

Description:
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Q5? High-Fidelity DNA Polymerase is an ultra high-fidelity,
Thermo
stable DNApolymerase with 3?→ 5? exonuclease activity, fused to a processivity-enhancingSso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymeraseis ideal for cloning and can be used for long or difficult amplicons. The Q5High-Fidelity 2X Master Mix offers robust, high-fidelity performance in aconvenient master mix format. The mix contains dNTPs, Mg
++
and a proprietarybroad-use buffer requiring only the addition of primers and DNA template forrobust amplification, regardless of GC content. When used at the recommended1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl
2
.
Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.To determine the optimal annealing temperatures for a given set of primers,use of the
NEB
Tm Calculatoris highly recommended.
This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions (
NEB
#E0555S) or 200 reactions (
NEB
#E0555L). For added convenience, gel lo
ADI
ng dye, and a Quick-Load DNA LAdder are also included in this kit.
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Kit Components
The following reagents are supplied with this product:
Store at (°C)
Concentration
Q5? High-Fidelity 2X Master Mix
-20
2X
Nuclease-free Water
-20
Quick-Load? Purple 2-Log DNA Ladder (0.1 - 10.0 kb)
4
Gel Lo
ADI
ng Dye, Purple (6X)
25
6X
Notes:
Product specifications for individual components in the Q5 High-Fidelity PCR Kit are available separately.