商品编号


M0330S-200 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




200 units


Concentration

5,000 units/ml

商品信息

Description:
Bsu
DNA Polymerase I, Large Fragment retains the 5?→ 3? polymerase activity of the
Bacillus

subtilis
DNA polymerase I (1), but lacks the 5?→ 3? exonuclease domain. This large fragment naturally lacks 3?→ 5? exonuclease activity.
Product Source
An?
E. coli
?strain that contains a genetic fusion of the?
Bacillus subtilis
?DNA polymerase I gene (starting from codon 297 thus lacking the 5?→ 3? exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved off?
in vitro
. The remaining DNA polymerase is purified free of MBP.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 2
-20
10X
Notes:
Bsu DNA Polymerase, Large Fragment is not suitable for generating blunt ends because it lacks the 3?→ 5? exonuclease necessary to remove non-templated 3? additions.Bsu DNA Polymerase, Large Fragment retains 50% activity at 25°C and is twice as active as Klenow Fragment (3?→ 5? exo–) at this temperature.