商品编号


R0559S-50 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

50 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the cloned BsgI gene from
Bacillus sphaericus
B922
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
S-adenosylmethionine (SAM)
-20
32 mM
CutSmart? Buffer
-20
10X
Notes:
BsgI requires 80 ?M S-adenosyl-methionine in reaction mixture for optimal activity (supplied with enzyme). Incubation without S-adenosylmethionine results in 25% activity.Storage of SAM: S-adenosylmethionine is stored at –20°C as 32 mM solution dissolved in sulfuric acid (0.005 M) and 10% ethanol. SAM in this solution stored under ideal conditions remains active for up to 6 months. SAM is unstable at (pH 7.5), 37°C, and should be replenished for reactions incubated longer than 4 hours. Many problems in achieving complete digestion can be alleviated by using fresh SAM.SAM should be kept frozen at shipping concentration and diluted prior to each reaction.