商品编号


R0711S-250 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

2,000 units/ml


Size

250 units

商品信息

Description:
Product Source
An
E.coli
strain that carries the cloned NmeAIII gene from
Neisseria meningitidis
2491 (Achtman, M.)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
S-adenosylmethionine (SAM)
-20
32 mM
Notes:
Overdigestion with > 5 units of NmeAIII per ?g of DNA is not recommended.Requires S-adenosylmethionine for optimal activity (supplied with enzyme)NmeAIII requires two copies of its recognition sequence for cleavage to occur. Thus, the single NmeAIII site in pUC19 is resistant to cleavage. A 10-fold overdigestion cuts less than half of the DNA.The cleavage point may shift one base pair depending on the DNA sequence context between the recognition site and the position of cleavage. For a given sequence, generally one cut site will predominate.Significant cleavage occurs on ice and at 25°C.?NmeAIII produces a stable partial digestion pattern even with excess enzyme. 1 unit is defined as the amount of enzyme required to produce this stable partial digestion pattern.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.