商品编号


R0501S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

500 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the RsrII gene from
Rhodopseudomonas sphaeroides
(S. Kaplan).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
RsrII requires two copies of its recognition sequence for efficientcleavage to occur. If a DNA molecule (plasmid or linear) has only onecopy of this recognition site, RsrII cannot digest it to completion.These two copies of recognition sites should ideally be on the samemolecule to achieve 100% cleavage, however, two different molecules canbe used with slightly less efficiency. For more information andrecommendations on working with enzymes requiring multi-sites such asRsrII, please visit Restriction Enzyme Cleavage: ‘single-site’ enzymes and ‘multi-site’ enzymes