商品编号


3253/10



品牌


Tocris



公司


Bio-Techne Corporation



公司分类


Polymerases



Size

10 mg

商品信息

Description:
Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive

Chemical Name:
(3b
S
,4a
S
,5a
S
,6
R
,6a
R
,7a
S
,7b
S
,8a
S
,8b
S
)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)
tris
oxireno[4
b
,5:6,7:8
a
,9]phenanthro[1,2-
c
]furan-1(3
H
)-one

Purity:
≥98% (HPLC)


BIOLOG
ical Activity Technical Data Solubility Calculators Datasheets References


BIOLOG
ical Activity

Inhibits DNA-dependent ATPase activity of XBP and induces inhibition of RNA polymerase II (RNAPII)-mediated transcription (IC
50
= 200 nM). Selective for RNAPII over RNAPI and RNAPIII. Blocks RNA synthesis in HeLa cells (IC
50
= 62 nM); exhibits potent antiproliferative activity in 60 cancer cell lines (average IC
50
= 12 nM) and induces apoptosis by blocking TNF-
α
-mediated c-IAP1 and c-IAP2 induction. Also displays immunosuppressive and anti-inflammatory activity.



Compound Libraries

Triptolide is also offered as part of the
Tocris
creen Plus and
Tocris
creen Epigenetics Toolbox. Find out more about compound libraries available from
Tocris
.



Technical Data



M. Wt

360.4



Formula

C
20
H
24
O
6




Storage

Store at -20°C



Purity

≥98% (HPLC)



CAS Number

38748-32-2



PubChem ID

107985



InChI Key

DFBIRQPKNDILPW-CIVMWXNOSA-N



Smiles

O=C1C2=C([C@@](C[C@H]5[C@]4(O5)[C@](O6)3[C@@H]6[C@H]7[C@](C(C)C)(O7)[C@H]4O)([H])[C@]3(C)CC2)CO1



The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All
Tocris
products are intended for laboratory research use only.



Solubility Data




Solvent

Max Conc. mg/mL

Max Conc. mM



Solubility



DMSO

7.21

20





Preparing Stock Solutions

The following data is based on the
product
molecular weight
360.4
. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.



Concentration / Solvent Volume / Mass

1 mg

5 mg

10 mg



1 mM

2.77 mL

13.87 mL

27.75 mL



5 mM

0.55 mL

2.77 mL

5.55 mL



10 mM

0.28 mL

1.39 mL

2.77 mL



50 mM

0.06 mL

0.28 mL

0.55 mL






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Product Datasheets


Certificate of Analysis / Product Datasheet


Safety Datasheet

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References

References are publications that support the products'
BIOLOG
ical activity.


Qui

et al
(1999) Immunosuppressant PG490 (Triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-κB transcriptional activation. J.Biol.Chem.
274
13443 PMID: 10224109


Lee

et al
(1999) PG490 (Triptolide) cooperates with tumor necrosis factor-α to induce apoptosis in tumor cells. J.Biol.Chem.
274
13451 PMID: 10224110


Titov

et al
(2011) XPB, a subunit of TFIIH, is a target of the natural product triptolide. Nat.Chem.Biol.
7
182 PMID: 21278739




Keywords: Triptolide, supplier, Blocks, TNF-α, TNF-alpha-mediated, c-IAP2, c-IAP1, induction, inhibitors, inhibits, apoptosis, TNF-alpha-induced, NF-κB, NF-kappaB, NF-kB, activation, X-Linked, Apoptosis, XIAP, Nuclear, Factor, Kappa, B, Cytokine, Signaling, Signalling, Transcription, Factors, Immunosuppressants, RNA/DNA, Polymerase, RNA, Polymerase, DNA,, RNA, and, Protein, Synthesis, Inhibitor, of, Apoptosis, (IAP), RNA/DNA, Polymerase,
Tocris
Bioscience