商品编号


H0250S



品牌


Biohelix



公司


BIO-HELIX Co., LTD.



公司分类


Helicases



Size

50 ?g


Concentration

1 mg/ml








商品信息



Description:


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The T7 Gp4A Helicase exhibits a 5? to 3? helicase activity as well as primase activity. It is highly processive, traveling an average of 75 kb of single stranded DNA before dissociating.


SUPPLIED WITH:
10X Gp4A Reaction Buffer


Supplied by Users
: dTTP




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Source:


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Purified from an
E. coli
strain that overexpresses the
gp4A
gene isolated from the T7 phage.



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Reaction condition:


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1X Gp4A Reaction Buffer:
35 mM Tris?acetate, pH 7.5
11 mM magnesium acetate
5 mM DTT
0.01% Triton X-100

Supplement with 2 mM dTTP.
Incubate at 25 - 37?C.

NOTE:


The concentration of dTTP should be optimized for each specific application.

For the primase activity, supplement reactions with NTPs.






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Storage Conditions:


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Storage Temperature:
-20?C


Concentration:

1 mg/ml

Storage Buffer:

20 mM TrisHCl pH 7.5
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.5 @ RT




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Quality Control:


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Quality Assurance Statement:

T7 Gp4A Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity in functional activity assays.




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