商品编号


M0289S-5,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


DNA Modifying Enzymes



Concentration

5,000 units/ml


Size

5,000 units

商品信息

Description:
Antarctic Phosphatase (AnP) is a heat l
ABI
le alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5? and 3? ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular
BIOLOG
y applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5? protruding, 5? recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irrevers
IBL
y inactivated by heating at 80°C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.?
Product Source
An?
E. coli
?strain that carries the TAB5 AP gene, originally cloned in plasmid pNI (2), recloned in plasmid pEGTAB7-4.1(3).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Antarctic Phosphatase Reaction Buffer
-20
10X
Notes:
Adding 1/10 volume of 10X Antarctic Phosphatase Reaction Buffer will provide the amount of Zn2+?that the enzyme requires for activity.