商品编号


M0535S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




500 units


Concentration

2,000 units/ml

商品信息

Description:
High Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at
New England Biolabs
,
Thermo
Scientific
? Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel
Pyrococcus
-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate 50-fold lower than that of
Taq
DNA Polymerase and 6-fold lower than that of
Pyrococcus furiosus
DNA Polymerase (1), Phusion is one of the most accurate
Thermo
stable polymerases available. Phusion DNA Polymerase possesses 5?→ 3? polymerase activity, 3?→ 5? exonuclease activity and will generate blunt-ended products.
Phusion Hot Start Flex DNA Polymerase offers robust, high fidelity performance and room temperature reaction setup. The addition of an aptamer-based inhibitor allows room temperature reaction setup. Phusion Hot Start Flex DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl
2
(1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl
2
solutions.
Phusion Hot Start Flex DNA Polymerase is unlike other enzymes and care must be taken when designing cycling protocols. To determine the optimal annealing temperatures for a given set of primers, use of the
NEB
T
m
Calculatoris highly recommended.
Product Source
An?
E. coli?
strain that carries the Phusion DNA Polymerase gene.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
MgCl
2
solution
50 mM
DMSO
100%
Phusion? HF Buffer Pack
-20
5X
Phusion? GC Buffer Pack
-20
5X