商品编号


M0203S-750 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




750 units


Concentration

3,000 units/ml

商品信息

Description:
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5?→ 3? direction and requires the presence of template and primer. This enzyme has a 3?→ 5? exonuclease activity which is much more active than that found in DNA Polymerase I (
E. coli
). ?Unlike
E. coli
DNA Polymerase I, T4 DNA Polymerase does not have a 5?→ 3? exonuclease function.
Highlights
Extreme fidelity (6)
Gap filling (no strand displacement activity)
Best enzyme for creating blunt ends
Isolated from a recombinant source
Product Source
Purified from a strain of?
E. coli
?that carries the T4 DNA Polymerase gene.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 2.1
-20
10X
Notes:
For fill-in reactions only: T4 DNA Polymerase can be used in
NEB
uffers 1.1, 2.1, 3.1 and CutSmart??Buffer as well as
NEB
uffers 1-4 and T4 DNA Ligase Reaction Buffer.For blunting reactions requiring removal of overhangs: T4 DNA Polymerase can be used in
NEB
uffers 1.1, 2.1, and CutSmart Buffer as well as
NEB
uffers 1, 2, and 4 and T4 DNA Ligase Reaction Buffer.
NEB
uffers 3.1 and 3 are not recommended when overhang removal is required. Optimal activity is observed in
NEB
uffer 2.1.Supplement with dNTPs.*BSA supplementation is recommended when using a buffer that does not already contain BSA. Incubate at temperature suggested for specific protocol.*Refer to specific protocol to determine recommended dNTP concentrations.