商品编号


R0545S-1,250 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

2,000 units/ml


Size

1,250 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the BcgI gene from
Bacillus coagulans
(H. Kong).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
S-adenosylmethionine (SAM)
-20
32 mM
NEB
uffer? 3.1
-20
10X
Notes:
BcgI cleaves DNA substrates twice to excise its recognition site generating a 32 base-pair fragment with 2-base 3? overhangs.Requires S-adenosylmethionine for optimal activity (supplied with enzyme). S-adenosylmethionine is stored at -20°C as a 32?mM solution dissolved in sulfuric acid (0.005?M) and 10% ethanol. SAM in this solution stored under ideal conditions remains active for up to 6?months. SAM is unstable at pH 7.5 and 37°C and should be replenished for reactions incubated longer than 4 hours. Many problems in achieving complete digestion can be alleviated by using fresh SAM.May exhibit star activity in
NEB
uffer 2.1, or CutSmart Buffer.