商品编号


EG-161



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


DNA Modifying Enzymes



Size

2,000 Units

商品信息



Description


T4 Endonuclease V?(T4 pyrimidine dimer glycosylase,?T4 PDG), has both DNA N-glycosylase and apurinic/apyrimidinic lyase (AP lyase) activities. This enzyme ?recognizes ?cis-syn-cyclobutane pyrimidine dimers in DNA caused by ultraviolet (UV) irr
ADI
ation, and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.




Applications



Study of UV damage to DNA and its repair, including DNA damage in single cells

Single cell gel electrophoresis (comet assay)





Source

Purified from an E. coli strain carrying a plasmid encoding T4 denV gene.



Components


T4 Endonuclease V
: 10,000 units/ml in 10 mM Tris-HCl, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25°C





Unit Definition


One unit of T4 Endonuclease V converts 1 ?g of UV-irr
ADI
ated plasmid DNA from covalently closed circles to nicked form in 30 minutes at 37°C.



Quality Control


The absence of nonspecific exo- and endonuclease activities and RNase activity is confirmed by appropriate quality test.




Storage Condition


-20 °C