商品编号


EG-1012



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


DNA Modifying Enzymes



Size

1,000 Units

商品信息



Description


Lam
BD
a Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex DNA. Lam
BD
a exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lam
BD
a Exonuclease exhibits low activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lam
BD
a exonuclease has no activity at nicks and limited activity at gaps in DNA




Applications



Generating single-stranded PCR products for use in:

DNA sequencing

Analysis of DNA single-strand conformation polymorphism (SSCP)

Rolling circle amplification

Producing single-stranded DNA from double-stranded DNA fragments

Cloning of PCR products





Source

An
E. coli
strain that carries the
Phage lam
BD
a exo
gene



Unit Definition


One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C




Components



Lam
BD
a Exonuclease:
5,000 U/ml in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM DTT, 0.1% mM EDTA, 50% Glycerol, pH 7.5 @ 25°C

10X Reaction Buffer
:
670 mM Glycine-KOH, 25 mM MgCl2, pH 9.4 @ 25°C





Quality Control


The absence of endodeoxyribonucleases is confirmed by appropriate quality test.
Functionally tested for generating single-stranded DNA from double-stranded PCR product.




Storage Condition


-20 °C