商品编号


EG-1034



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




4,000 Units

商品信息



Description

Thermo
stable
Taq
DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD
+
as a cofactor.



Applications



Ligation of DNA fragments at elevated temperatures (45°C-65°C).

Ligase Chain Reaction.

Gibson Assembly of DNA fragments (Gene Synthsis, joning multiple large DNA fragments).





Source

Purified from a recombinant
E. coli
strain carrying the cloned
Taq
DNA Ligase gene.



Components



Taq
DNA Ligase, 40 U/?l in storage buffer: 10 mM Tris-HCl, 50 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Tween-20 50% Glycerol, pH 7.5 @ 25°C.

10X
Taq
DNA Ligation Buffer: 200 mM Tris-HCI, 250 mM KCl, 100 mM MgCl
2,
5 mM NAD, 0.1% Triton X-100, pH 7.6 @ 25°C





Unit Definition

1 unit is defined as the amount of
Taq
DNA Ligase required to join 50% of 1 ?g of the 12-base cohesive ends of Lam
BD
a DNA cut with Hind III in 50 ?l 1X
Taq
DNA Ligase Buffer following a 10 minute incubation at 45°C.



Quality Control



The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases confirmed by appropriate quality tests.

Functionally tested for the capacity to join cohesive- and blunt-end DNA fragments.





Storage

-20°C.