商品编号


EG-1051



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




10,000 Units

商品信息



Description

T4 RNA Ligase 1 catalyzes the ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA)



Applications



Labeling of 3'-termini of RNA with 5'-[
32
P] pCp

Inter- and intramolecular joining of RNA and DNA molecules

Synthesis of single-stranded oligodeoxyribo-nucleotides

Incorporation of unnatural amino acids into proteins

Ligation of ss-RNA and DNA





Source

Purified from a recombinant
E. coli
strain carrying the T4 phage RNA Ligase 1 gene



Components



T4 RNA Ligase 1
: 20,000 U/ml in storage buffer: 10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1.0 mM DTT, 50% glycerol, pH 7.5 @ 25°C

10 X T4 RNA Ligase Buffer:
500 mM Tris-HCl, 100 mM MgCl
2
, 10 mM ATP, 100 mM DTT, pH 7.8 @ 25°C





Unit Definition

One unit is defined as the amount of enzyme required to ligate 50% of 0.4 ?g of an equimolar mix of two single stranded 23 base RNA oligonucleotides (one 5′-phosphorylated) in 20 ?L 1X T4 RNA Ligase Buffer following a 30 minute incubation at 37°C



Quality Control



The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases confirmed by appropriate quality tests.

Functionally tested for the capacity to join cohesive- and blunt-end DNA fragments.





Storage

-20°C.