商品编号


EG-146



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Ligases




10,000 Units

商品信息



Description


ATP independent T4 RNA Ligase 2 specifically ligates the preadenylated 5? end of DNA or RNA to the 3? OH end of RNA. The enzyme does not require ATP for ligation but requires pre-adenylated linkers.




Applications



Ligate a pre-adenylated DNA or RNA sequence tag to any RNA 3?-end

Join a single stranded adenylated primer to small RNAs for
CDN
A library creation

Join a single stranded adenylated primer to RNA for strand-specific
CDN
A library construction.





Source

Purified from a recombinant
E. coli
strain carrying a mutant of T4 RNA Ligase 2.



Components



ATP independent T4 RNA Ligase 2
, 200,000 ? units/ml in storage buffer: 10 ml Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% Glycerol, pH 7.5 @ 25°C

10 X T4 RNA Ligase 2 truncated Buffer:
500 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.5 @ 25°C





Unit Definition


200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA in a total reaction volume of 20 μl in 1 hour at 25°C.

5?-FAM- rArGrUrCrGrUrArGrCrCrUrUrUrArUrCrCrGrArGrArUrUr CrArGrCrArArUrA-3?
5?-rAppCTGTAGGCACCATC
AAT
–NH2-3?




Quality Control


The absence of endo-, exodeoxyribonucleases, phosphatase and ribonucleases confirmed by appropriate quality tests.




Storage

-20°C.