商品编号


EG-1056



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




2,500 Units

商品信息



Description


Klenow Fragment (3?→ 5? exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5?→ 3? exonuclease (nick-translation) activity, and proofre
ADI
ng (3′→ 5′) exonuclease activity due to two amino acids mutations (D355A, E357A).




Applications



Random-primed DNA labeling

DNA sequencing by the Sanger dideoxy method

Second strand
CDN
A synthesis

Labeling by fill-in 5'-overhangs of dsDNA

Strand displacement amplification (SDA)





Source

A recombinant
E. coli
strain carrying the mutated Klenow Fragment of
E coli
PolA gene.



Unit Definition


One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.




Components



Klenow Fragment exo-:
5,000 U/ml in 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C

10X Reaction Buffer
: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl
2
, 10 mM DTT, pH 7.9 @ 25°C





Quality Control



The absence of endo- and exodeoxyribonucleases confirmed by appropriate quality tests.

Functionally tested in random-primed DNA labeling.





Storage Condition


-20 °C