商品编号


EG-1057



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




10,000 Units

商品信息



Description

φ29 DNA Polymerase is a highly processive polymerase from
Bacillus Subtilis
phage φ29. It has exceptional strand displacement and processive synthesis properties, which allows for highly efficient isothermal DNA amplification. The enzyme possesses a 3'→5' exonuclease (proofre
ADI
ng) activity acting preferentially on single-stranded DNA, allowing high-fidelity DNA synthesis.



Applications



Rolling circle amplification (RCA): generation of periodic DNA nanotemplates

Multiple displacement amplification (MDA)

Unbiased amplification of whole genome (WGA):

amplification of DNA for SNP and STR detection

cell-free amplification of DNA from single cells

pathogenic organisms or metagenomes

amplification of DNA from filter paper blood spot samples



DNA template preparation for sequencing

Protein-primed DNA amplification

In situ
genotyping with padlock probes

Recombination based-cloning

Cell-free cloning of lethal DNA

RNA-primed DNA amplification





Source

A recombinant
E. coli
strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29



Unit Definition

One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.



Components



phi29 DNA Polymerase:
100,000 U/ml in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C

10X Reaction Buffer
: 500 mM Tris-HCl, 100 mM (NH
4
)
2
SO
4
, 40 mM DTT, 100 mM MgCl
2
, pH 7.5 @ 25°C





Quality Control

The absence of endodeoxyribonucleases confirmed by appropriate quality tests.



Storage Condition

-20 °C