商品编号


EG-1059



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




5,000 Units

商品信息



Description


T7 DNA Polymerase catalyzes DNA synthesis in the 5'->3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a very strong 3'->5' exonuclease activity towards single- and double-stranded DNA.




Applications



Purification of covalently closed circular DNA by removal of residual genomic DNA

Primer extension reactions on long templates

DNA 3'-end labeling

Strand extensions in site-directed mutagenesis

Fill-in blunting of 5'-overhang DNA

Second strand synthesis of
CDN
A

In situ
detection of DNA fragmentation associated with apoptosis





Source

T7 DNA Polymerase is composed of two subunits: an 80 kDa polypeptide (the product of gene 5 of bacteriophage T7) and a 12 kDa thioredoxin from the
trxA
gene of
E. coli
. Each protein is cloned and overexpressed separately in
E. coli
.



Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.



Components



T7 DNA Polymerase:
10,000 U/ml in 50 mM KPO
4
, 0.1 mM EDTA, 1.0 mM DTT, 50% glycerol, pH 7.0 @ 25°C

10X Reaction Buffer
: 200 mM Tris-HCl, 100 mM MgCl
2
10 mM DTT, pH 7.5 @ 25°C





Quality Control

The absence of endodeoxyribonucleases is confirmed by the appropriate quality test.



Storage Condition

-20 °C