商品编号


EG-1060



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




50,000 Units

商品信息



Description

T7 RNA Polymerase catalyzes the 5'->3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from a T7 phage promoter (
T
AAT
ACGACTCACTATA
G
GGAGA
).



Applications



R
ADI
olabeled RNA probe preparation

RNA generation for
in vitro
translation

RNA generation for studies of RNA structure, processing and catalysis

Expression control via anti-sense RNA





Source

Purified from a strain of
E. coli
that expresses the recombinant T7 RNA Polymerase gene (gene I)



Unit Definition

One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.



Components



T7 RNA Polymerase:
50,000 U/ml in 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C

10X Reaction Buffer
: 400 mM Tris-HCl, 60 mM MgCl
2
, 100 mM DTT, 20 mM, Spermidine, pH 7.9 @ 25°C





Quality Control



The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate tests.

Functionally tested in in vitro transcription reaction.





Storage Condition

-20 °C