商品编号


EG-151



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




100 Units

商品信息



Description


S. pombe
Poly(U) (Poly(A)) Polymerase uses ATP as a substrate for template-independent addition of UMP from UTP or AMP from ATP to the 3? hydroxyl termini of RNA molecules.




Applications



Labeling of RNA with UTP

Poly(U) and Poly(A) tailing of RNA for cloning

Enhance mRNA st
ABI
lity and increase translation efficiency of in vitro synthesized mRNA transferred into eukaryotic cells

Poly (A) tailing of 2' O-Me modified 3' ends





Source

A recombinant
E. coli
strain carrying yeast
(
Schizosaccharomyces pombe
)
Poly(A) Polymerase gene Cid1



Unit Definition

1 unit is defined as the amount of enzyme that will incorporate 1 nmol of UMP into into RNA in 50 ?l in 10 minutes at 37°C.



Components



S. pombe

Poly(U) Polymerase:
2,000 U/ml in 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton? X-100, pH 7.5 @ 25°C

10X Reaction Buffer
: 100 mM Tris-HCl,500 mM NaCl, 100 mM MgCl2,10 mM DTT, pH 7.9 @ 25°C





Quality Control

The absence of endonuclease, nicking activity,

exonuclease and RNAse

activity is confirmed by appropriate quality tests.



Storage Condition


-20 °C