商品编号


EG-200



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Polymerases




20,000 Units

商品信息



Description


Gst DNA Polymerase is

functionally equivalent to the large fragment of Bsm Polymerase I and Bst Polymerase I. Recombinant Gst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease and 3'→5' exonuclease activities
. Gst DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications such as
rapid amplification of trace amounts of DNA templates for sequencing and LAMP
.




Applications



Isothermal DNA amplification by the method of:

Loop-mediated isothermal amplification (LAMP).

Whole genome amplification (WGA).

Ramification amplification (RAM).



Random-primed DNA labeling

Labeling by fill-in 5'-overhangs of dsDNA





Source

A recombinant
E. coli
strain carrying the large fragment of DNA polymerase gene from
Geobacillus stearo
Thermo
philus



Unit Definition

One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.



Components



Gst DNA Polymerase:
100,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C

10X Reaction Buffer
: 200 mM Tris-HCl, 100 mM (NH
4
)
2
SO
4
, 100 mM KCl, 20 mM MgSO
4
, and 1.0 % Triton X-100, pH 8.8 @ 25°C





Quality Control

The absence of endonuclease, nicking activity and

exonuclease

activity is confirmed by appropriate quality tests.



Storage Condition


-20 °C