商品编号


EG-208



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Restriction Enzymes



Size

300 rxns

商品信息



Recognition Sequence


5'...C A↓T A T G...3'
3'...G T A T↑A C...5'




Components



NdeI
, 1 reaction/?l in storage buffer containing 50% glycerol.

10 X FastCut Buffer





Activity

1 μl of enzyme cleaves 1 μg of lam
BD
a DNA in 10 minutes at 37°C in 1X FastCut Buffer.



Quality Control



The absence of nicking endodeoxyribonucleases, exodeoxyribonucleases, and nonspecific DNases activities is confirmed by appropriate quality tests.

Functionally tested for the capacity to digest lam
BD
a DNA, plasmid DNA, PCR products fragments, and ligation and recutting assay.





Recommended Reaction Conditions



2 ~3 μl of 10X FastCut Buffer

~ 1 μg Plasmid DNA or ~200 ng PCR product

ddH2O to 19 or 29 μl

1 μl enzyme

Incubate at 37 °C for 10 min for plasmid or 60 min for PCR product.





Storage

-20°C.