商品编号


TAB1R80524



品牌


Lucigen



公司


Lucigen



公司分类


RNA Amplification and Labeling



Size

24 Reactions


商品信息

Optimized for high yield, high quality biotin-aRNA

Highly reproduc
IBL
e amplification, labeling, and microarray results.

Multiple RNA samples can be easily amplified and labeled simultaneously.

Linear RNA amplification process preserves the transcript profile of the sample.

Simple, single-tube, 6-hour procedure. Prepare biotin-aRNA and begin hybridization the same day.

Biotin-aRNA produced by the kit detects more transcripts (genes) than competitor kits and showed high correlation with MAQC TaqMan? data (Fig. 1).




Applications

The TargetAmp? 1-Round Biotin-aRNA Amplification Kit 105* produces microgram amounts of biotin-antisense RNA (biotin-aRNA, also called biotin-cRNA) from as little as 25 ng of total cellular RNA. The kit provides all reagents for the preparation of biotin-aRNA (except reverse transcriptase and RNA purification columns) and uses an improved Eberwine-type linear amplification method
1
to achieve at least 5,000-fold amplification of the poly(A) RNA present in a total cellular RNA sample. The entire procedure can be completed in 1 day.

Table 1. A TargetAmp? Kit 105 reaction produces high yields of biotin-aRNA from as little as 25 ng of input total RNA.
Results represent the average of multiple experiments.


Amount of Input HeLa Total RNA

Biotin-aRNA Yield from HeLa Total RNA
a


25 ng

4.1 ?g


100 ng

13.7 ?g


500 ng

64.9 ?g



a. Approximately 2% of HeLa total RNA is poly(A) RNA.




Figure 1. Correlation of expression ratios obtained from qPCR and from microarrays hybridized to aRNA prepared with the TargetAmp? 105 kit.
X axis, log
2
expression ratios determined from Human Genome U133 Plus 2.0 Arrays (
Affymetrix
) hybridized to targets prepared by the TargetAmp 105 kit; Y axis, log
2
expression ratios determined by the MAQC, using TaqMan? assays. The high correlation obtained (r = 0.936) indicates the excellent fidelity of the amplification process.



References

Van Gelder, R.N.
et al
. (1990)
Proc. Natl. Acad. Sci. USA

87
, 1663.


*
TargetAmp? aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.

For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.

ORDER INFORMATION
These kits were optimized using SuperScript? III reverse transcriptase (
Invitrogen
), which is not included in the kits. Contents: TargetAmp? T7-Oligo(dT) Primer A, TargetAmp? Reverse Transcription PreMix, TargetAmp? DNA Polymerase PreMix I, TargetAmp? DNA Polymerase, TargetAmp? T7 RNA Polymerase, TargetAmp? T7 Transcription Buffer, TargetAmp? NTP PreMix, TargetAmp? UTP / Biotin-UTP, RNase-Free DNase I, DTT, HeLa Total RNA Control, RNase-Free Water.