商品编号


TAN091096



品牌


Lucigen



公司


Lucigen



公司分类


RNA Amplification and Labeling



Size

96 Reactions

商品信息

Optimized for high signal intensity

Fast.
Perform the 6-hour reaction and begin BeadChip hybridization the same day.

High Sensitivity.
Sensitive and reproduc
IBL
e gene detection.

High Yield.
Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 25 ng of total RNA.

High Fidelity.
TargetAmp-Nano labeling procedure preserves the relative abundance of the original sample.




The TargetAmp?-Nano Labeling Kit produces biotin-aRNA (also called biotin-cRNA) that has been optimized for high signal intensity on
Illumina
Expression BeadChip arrays, enabling sensitive gene detection and reproduc
IBL
e differential expression results.

Benefits

Fast.
Perform the 6-hour reaction and begin BeadChip hybridization the same day.

High Sensitivity.
Sensitive and reproduc
IBL
e gene detection.

High Yield.
Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 25 ng of total RNA.

High Fidelity.
TargetAmp-Nano labeling procedure preserves the relative abundance of the original sample.

Table 1. TargetAmp-Nano Kit aGuide



Input RNA

Reaction
Time

Additional

TargetAmp-Nano Kit

25 ng - 500 ng total RNA

6 hours

Requires use of SuperScript III Reverse Transcriptase
(provided by the user)



Table 2. Yield of Biotin-aRNA from TargetAmp-Nano Kit

Input RNA (HeLa total RNA)

50 pg

100 pg

500 pg

25 ng

100 ng

500 ng

TargetAmp-Nano Kit reaction







3 μg

10 μg

75 μg


ORDER INFORMATION
These kits were optimized using SuperScript? III reverse transcriptase (
Invitrogen
), which is not included in the kits. Contents: TargetAmp? T7-Oligo(dT) Primer, TargetAmp? Nano 1st-Strand
CDN
A Premix, TargetAmp? Nano 2nd-Strand
CDN
A Premix, TargetAmp? Nano 2nd-Strand
CDN
A DNA Polymerase, TargetAmp? Nano T7 RNA Polymerase, TargetAmp? Nano T7 Transcription Buffer, TargetAmp? Nano NTP PreMix, TargetAmp? Nano UTP / Biotin-UTP, RNase-Free DNase I, DTT, HeLa Total RNA Control, RNase-Free Water.