商品编号


RJ411250



品牌


Lucigen



公司


Lucigen



公司分类


DNA Nucleases




250 Units

商品信息

Magnesium-dependent digestion of single-stranded DNA in a 5? to 3? direction

Specifi
c: Digests ssDNA in the 5' to 3 direction

Heat Inactivated
: Heat at 65°C for 20 minutes to kill enzyme activity

Flex
IBL
e
: Use to remove primers from completed PCR or to degrade single-stranded linear DNA in dsDNA and plasmid preps




Rec J Exonuclease, derived from
E. coli
, catalyzes degradation of ssDNA in a 5? to 3? direction. Its activity is dependent on Mg
2+
. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

Applications


Removal of primers from completed PCR.

Degradation of single-stranded linear DNA in dsDNA and plasmid preps.


Unit Definition:
One unit of Rec J Exonuclease catalyzes the release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37°C under standard assay conditions.

Storage Buffer:
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton? X-100.

10X Reaction Buffer:
330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT.

Quality Control:
Rec J Exonuclease is free of detectable RNase and contaminating DNA endonuclease activities.

ORDER INFORMATION
Includes 10X Reaction Buffer.