商品编号


30241-1



品牌


Lucigen



公司


Lucigen



公司分类


Modifying Enzymes



Size

1,500 U



商品信息



Reliably join double-stranded nucleic acid chains.





T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.



T4 DNA Ligase Version

Sizes

Ligase Concentration

Provided Buffer



Low Concentration Ligase

1,500 U
7,500 U

2 U/?L

10X T4 DNA Ligase Buffer



High Concentration Rapid Kit

1,500 U
7,500 U

10 U/?L

2X Rapid Ligation Buffer,
10X T4 DNA Ligase Buffer




10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl
2
, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.

2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl
2
, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.

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Figure 1:? Purity
The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.? Molecular weight
Marker
s are in Lane 1.? T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).












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Figure 2:?
Exonuclease and Endonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 ?g of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).? Molecular weight
Marker
s are in Lanes 1 and 2.






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Unit Definition:
One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of
32
P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.

Source:
A recombinant
E. coli
strain carrying the cloned T4 DNA Ligase gene.

ORDER INFORMATION
Storage buffer:
T4 DNA Ligase is supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1%Triton X-100, 50% glycerol, pH 7.5 @ 25 °C.