商品编号


FC-132-1001



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
The Nextera Mate Pair Library Preparation Kit is an ideal approach for
de novo
sequencing, genome finishing, and the detection of structural variation. It is compat
IBL
e with large DNA genomes — even the most complex genomes such as those derived from cancer.


Sequence precious samples when limited DNA is available

Generate highly diverse libraries with premier data quality

Prepare sequencing-ready libraries in less than two days


Requires 10 Times Less DNA Input Than Other Protocols

Both gel-free and gel-plus protocols are available:


The gel-free protocol allows mate pair sequencing when limited DNA is available. It is designed for applications such as
de novo
assembly of small genomes.

The gel-plus protocol is ideal for challenging mate pair applications, such as
de novo
assembly of complex genomes.



Increased Library Diversity and Data Quality

The kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.


Only biotinylates DNA molecules at fragmentation sites, avoiding troublesome internal biotinylation

Allows for the creation of millions of unique fragments

Increased library diversity generates fewer duplicate reads and premier data quality


Fast and Simple Mate Pair Workflow

The Nextera Mate Pair kit uses TruSeq DNA Library Preparation master-mixed reagents, thereby reducing the number of assay steps and hands-on time required.


Fewer pipetting steps simplifies workflow and reduces sample loss

Gel-free protocol option and on-bead reactions simplify purification steps and shorten protocol hands-on time

Specifications:
Assay Time
1.5 - 2 days
Hands-On Time
1.5 - 2 hours
Input Quantity
1 ug DNA for gel-free protocol, 4 ug DNA for gel-plus protocol
Multiplexing
Can multiplex up to 12 samples, depending on genome size. Compat
IBL
e with most large DNA genomes.
Mechanism of Action
Methods that target the junction site of large circularized fragments. Mechanical fragmentation (
COVARIS
).
System Compatibility
MiSeq,HiSeq 3000,HiSeq 2500,HiSeq 4000
Species Category
Other,Mammalian,Mouse,Human,Rat,Plant,Bacteria
Variant Class
Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method
Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology
Sequencing