商品编号


T7DP-OEM



品牌


MCLab



公司


MCLAB.Inc



公司分类


Mesophilic DNA Polymerases



Size

Any Size

商品信息

T7 DNA Polymerase is the mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is respons
IBL
e for the rapid and accurate replication of the virus' genome during its infection cycle.




Description:
T7 DNA Polymerase is the mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is respons
IBL
e for the rapid and accurate replication of the virus genome during its infection cycle. T7 DNA Polymerase is a two subunit protein that consists of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (
E. coli
trxA gene thioredoxin)
(1, 2)
. The enzyme possesses a powerful (3' -> 5') nuclease activity that acts on both single and double stranded DNA and appears to be respons
IBL
e for the high fidelity of this enzyme and prevents strand displacement synthesis
(3,4,5)
.
Specific Activity:
10,000U/mg
Application:
Second strand synthesis in site-directed mutagenesis protocols
Source:
A recombinant
E. coli
strain carrying the bacteriophage T7 gene 5.
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of total dNTPs into acid insoluble material in 30 minutes at 37°C.
Supplied in:
50 mM KPO4
0.1 mM EDTA
1.0 mM Dithiothreitol
50% Glycerol
pH 7.0 @ 25°C
Supplied with:
10x T7 DNA Polymerase Buffer
10x T7 DNA Polymerase Buffer:
400 mM Tris-HCl
200 mM MgCl
2
500 mM NaCl
pH 7.5 @ 25°C
Recommended Storage Condition:
-20°C
Reference:
1. Grippo, P. et al. (1971) J. Biol. Chem. 246, 6867-6873
2. Modrich, P. et al. (1975) J. Biol. Chem. 250, 5515-5522
3. Adler, S. et al. (1979) J. Biol. Chem. 254, 11605-11614
4. Hori, K., et al. (1979) J. Biol. Chem. 254, 11598-11604
5. Lechner, R. L. et al. (1983) J. Biol. Chem. 258, 11185-11196