商品编号


BPL-200



品牌


MCLab



公司


MCLAB.Inc



公司分类


Mesophilic DNA Polymerases



Concentration

100 U/?l


Size

10,000 units

商品信息

For DNA sequencing high GC regions and rapid sequencing from nanogram amounts of DNA templates.




Description:
A recombinant
E. coli
strain carrying the BST DNA Polymerase gene (large fragment).
?


Figure:
MCLAB
Bst DNA Polymerase (large fragment) shows higher strand -displacing polymerase activity than two other vendors' products.


Application:
- Sequencing through problematic secondary structures
Source:
Bst DNA Polymerase (large fragment) is a portion of Bacillus stearo
Thermo
philus DNA Polymerase protein that contains 5?-> 3? polymerase activity, but lacks 5'->3' and 3'->5' exonuclease activities.
Specific Activity:
120,000 U/mg
Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C
Supplied with:
10X PCR buffer II
10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO
4
1.0% Triton X-100
pH 8.8 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Recommended Storage Condition:
-20°C
Reference:
Kiefer, et al. Structure 15 January 1997. 5, 95-108.