商品编号


FC-131-1002



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids


Rapid library preparation
– complete library prep in as little as 90 minutes with only 15 minutes of hands-on time

Fast time to results
– go from DNA to data in 8 hours with our benchtop sequencers.

Optimized for small genomes, PCR amplicons, and plasmids
– one library prep kit for many applications

Innovative sample normalization
– eliminates the need for library quantification before sample pooling and sequencing



With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including?small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded
CDN
A.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compat
IBL
e with all
Illumina
sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
90 minutes
Hands-On Time
15 minutes
Mechanism of Action
Enzymatic Fragmentation
Multiplexing
Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity
1 ng DNA
Species Category
Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility
MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method
Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class
Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types
Low Input,Single Cells
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots
Nextera XT Library Preparation Workflowimages/
Illumina
/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
The combination of Nextera XT and rapid sequencing with the MiSeq System provides a complete DNA to data workflow in only 8 hours.Innovative Sample Normalizationimages/
Illumina
/201710/nextera-xt-dna-library-prep-kit-web-graphic-2.png