商品编号


RPTK-200



品牌


MCLab



公司


MCLAB.Inc



公司分类


Reverse Transcriptase and RNA Polymerases



Size

100 reactions, 20 ?l/reaction

商品信息

The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.




Description:
The RNA Poly(A) Tailing Kit provides highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.
MCLAB
's newly engineered Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3?-hydroxyl termini of RNA molecules. Cloned Poly(A) Polymerase is encoded by an
E. coli
gene and overexpressed in the host strain.
Applications:
- Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand
CDN
A using a primer with poly(dT) on its 3? end.
- Addition of a poly(A) tail to RNA synthesized
in vitro
in order to increase the st
ABI
lity of the RNA and enhance its
ABI
lity to be translated
in vivo
after transfection or microinjection into eukaryotic cells.
- Synthesis of polyadenylated RNA for nucleic acid amplification methods or gene expression studies.
- 3?-end labeling of RNA or quantitate mRNA.
Recommended Storage Condition:
-20°C
References:
1. Drummond, D.R. et al. (1985) J. Cell. Biol. 100, 1148.
2. Galili, G. et al. (1988) J. Biol. Chem. 263, 5764.
3. Lingner, J. and Keller, W. (1993) Nucleic Acids Res. 21, 2917.