商品编号


PAP-OEM



品牌


MCLab



公司


MCLAB.Inc



公司分类


Reverse Transcriptase and RNA Polymerases



Size

Any Size

商品信息

Poly(A) Polymerase catalyzes the addition of AMP to the 3’ hydroxyl terminus of RNA. Poly(A) Polymerase can add poly(C) and poly(U) to RNA, but does so with much reduced efficiency.




Description:
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3'-hydroxyl of RNA. The reaction requires Mg
2+
and is template independent.
Application:
- Labeling of RNA with ATP or cordycepin
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells
Source:
The gene encoding
E. coli
Poly(A) Polymerase expressed in
E. coli
.
Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl
2
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C
Supplied with:
10x Poly(A) Polymerase Reaction Buffer
10x Poly(A) Polymerase Reaction Buffer:
500 mM Tris-HCl
2.5 M NaCl
100 mM MgCl
2
pH 7.9 @ 25°C
Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
Recommended Storage Condition:
-20°C