商品编号


53-1250



品牌


Minerva-biolabs



公司


Minerva-biolabs



公司分类


Enzymes



Size

1 Unit/μl 250 Units

商品信息

MB Taq DNA Polymerase is a highly processive 5?→3? polymerase with no 3?→5? exonuclease (proofre
ADI
ng) activity. At 72 °C and around pH 9 conditions, MB Taq DNA Polymerase reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds.
The Taq‘s activity is blocked at ambient temperature, e.g. while preparing your sample. The inhibition of the Taq polymerase is completely reversed when the temperature is above 70 °C. Complete activation of the polymerase is achieved at 94 °C for 2 min. MB Taq DNA Polymerase is supplied with a 10x buffer for maximal yield and sensitivity which should fit most standard applications.
Recommended Use / Scope
Routine and multiplex PCR; real-time PCR; T/A cloning; DNA labeling with biotin, digoxygenin or r
ADI
oactive nucleotids,; sequencing of double or single stranded DNA
Kit Components
MB Taq DNA Polymerase, supplied in 10 mM Tris-HCl (pH 7.0), 50 mM KCl, 0.1 mM EDTA, and 50 % (v/v) glycerol. 10x reaction buffer.
100 mM MgCl2 solution.
If the polymerase is used in combination with Venor?GeM or Venor?GeM Advance the buffer provided with the polymerase is not required.
Package Sizes
MB Taq DNA Polymerase available in concentrations of 1 u/?l and 5 u/?l.
1 Unit/?l
Cat. No. 53-1050
50 Units

Cat. No. 53-1100
100 Units

Cat. No. 53-1200
200 Units

Cat. No. 53-1250
250 Units
5 Units/?l
Cat. No. 53-0050
50 Units

Cat. No. 53-0100
100 Units

Cat. No. 53-0200
200 Units

Cat. No. 53-0250
250 Units
Shelf Life and Storage
Components must be stored at -18 °C. Shipment on cool packs. The product is stable for at least 6 months.