商品编号


FC-150-1002



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-le
ADI
ng accuracy. It offers:


Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing

Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads

Fully supported workflow solution to simplify exome sequencing


Cost-Effective Exome Sequencing

The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.


Proven TruSeq Data Quality

The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with
Illumina
systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina
provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert
Illumina
scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
2.5 days
Hands-On Time
6 hours
Input Quantity
100 ng genomic DNA
Content Specifications
Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action
Mechanical fragmentation (
COVARIS
) and exome enrichment with biotinylated capture probes
Multiplexing
Up to 12-plex enrichment
System Compatibility
NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000
Specialized Sample Types
FFPE
Technology
Sequencing
Species Category
Human
Automation Cap
ABI
lity
Liquid Handling Robots
Method
Exome Sequencing